Please use this identifier to cite or link to this item: http://115.74.233.203:81/tailieuso/handle/123456789/2219
Title: The effect of different media and temperature conditions for Salmonella bacteriophage preservation
Authors: Le, Nguyen Nam Phuong
Luu, Huynh Anh
Pham, Khanh Nguyen Huan
Huynh, Tan Loc
Chau, Thi Huyen Trang
Trinh, Thi Hong Mo
Nguyen, Hong Xuan
Nguyen, Trong Ngu
Keywords: Chăn nuôi & Thú Y
Glycerol
Medium
Phage
Storage
Temperature
Issue Date: 2022
Publisher: Veterinary Integrative Sciences
Citation: Anh, L. H., Huan, P. K. N., Loc, H. T., Trang, C. T. H., Mo, T. T. H., Xuan, N. H., & Ngu, N. T. (2022). The effect of different media and temperature conditions for Salmonella bacteriophage preservation: https://doi. org/10.12982/VIS. 2022.036. Veterinary Integrative Sciences, 20(2), 489-496.
Series/Report no.: Chăn nuôi & Thú Y;
Abstract: This research aimed to determine the optimal media and temperature conditions for the long-term storage of bacteriophages. In this study, the viability of Salmonella phages in 50% glycerol, 10% sodium chloride-magnesium sulfate (SM) buffer, and 5% dimethyl sulfoxide (DMSO) media at room temperature, 4oC, -20oC, and -80oC for 12 months was determined. In 50% glycerol, at the end of the experiment, no significant difference was found between four temperature conditions on phage density, ranging from 6.20-6.23 log10 PFU/mL (P>0.05). Under 10% SM medium, phage preservation at room temperature provided the optimum density at 6.31 log10 PFU/mL. In addition, phages preserved in a 5% DMSO medium were of similar density values across all temperature treatments. Still, their availability after 12 month-storage (88.0-88.5%) was significantly lower (P<0.05) than that of 50% glycerol and 10% SM. Moreover, for phage lysis capacity, low temperatures (4oC, -20oC, and -80oC) were superior to room temperature used for preservation. Considering the density, lysis capacity, and practical convenience, storing phages at 4°C in a 50% Glycerol medium is recommended.
URI: http://115.74.233.203:81/tailieuso/handle/123456789/2219
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