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http://115.74.233.203:81/tailieuso/handle/123456789/2219
Title: | The effect of different media and temperature conditions for Salmonella bacteriophage preservation |
Authors: | Le, Nguyen Nam Phuong Luu, Huynh Anh Pham, Khanh Nguyen Huan Huynh, Tan Loc Chau, Thi Huyen Trang Trinh, Thi Hong Mo Nguyen, Hong Xuan Nguyen, Trong Ngu |
Keywords: | Chăn nuôi & Thú Y Glycerol Medium Phage Storage Temperature |
Issue Date: | 2022 |
Publisher: | Veterinary Integrative Sciences |
Citation: | Anh, L. H., Huan, P. K. N., Loc, H. T., Trang, C. T. H., Mo, T. T. H., Xuan, N. H., & Ngu, N. T. (2022). The effect of different media and temperature conditions for Salmonella bacteriophage preservation: https://doi. org/10.12982/VIS. 2022.036. Veterinary Integrative Sciences, 20(2), 489-496. |
Series/Report no.: | Chăn nuôi & Thú Y; |
Abstract: | This research aimed to determine the optimal media and temperature conditions for the long-term storage of bacteriophages. In this study, the viability of Salmonella phages in 50% glycerol, 10% sodium chloride-magnesium sulfate (SM) buffer, and 5% dimethyl sulfoxide (DMSO) media at room temperature, 4oC, -20oC, and -80oC for 12 months was determined. In 50% glycerol, at the end of the experiment, no significant difference was found between four temperature conditions on phage density, ranging from 6.20-6.23 log10 PFU/mL (P>0.05). Under 10% SM medium, phage preservation at room temperature provided the optimum density at 6.31 log10 PFU/mL. In addition, phages preserved in a 5% DMSO medium were of similar density values across all temperature treatments. Still, their availability after 12 month-storage (88.0-88.5%) was significantly lower (P<0.05) than that of 50% glycerol and 10% SM. Moreover, for phage lysis capacity, low temperatures (4oC, -20oC, and -80oC) were superior to room temperature used for preservation. Considering the density, lysis capacity, and practical convenience, storing phages at 4°C in a 50% Glycerol medium is recommended. |
URI: | http://115.74.233.203:81/tailieuso/handle/123456789/2219 |
Appears in Collections: | Bộ sưu tập Bài báo tạp chí ngoài trường |
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